Bacterial nicotinate phosphoribosyltransferase (PncB) Assays

Nicotinate phosphoribosyltransferase (NAPRTase or PncB) provides a pathway of regenerating nicotinic acid adenine dinucleotide (NAD) through synthesizing nicotinic acid mononucleotide (NAMN). The enzyme transfers nicotinate (NA) onto phosphoribosyl pyrophosphate (PRPP) releasing pyrophosphate. PNCB is drug target for gram (+) bacteria.

The Bacterial Nicotinate Phosphoribosyltransferase (PncB) Assay is based on measurement of the pyrophosphate generated from the enzyme reaction.  The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of PNCB in drug discovery research.  It may also be used for characterization of PNCB enzyme.

S. pneumoniae PncB Assay Kit Plus-100 (Cat#. PNCB100KN)

S. pneumoniae PncB Assay Kit Plus-500 (Cat#. PNCB500KN)

S. aureus PncB Assay Kit Plus-100 (Cat#. PNCB100KS)

S. aureus PncB Assay Kit Plus-500 (Cat#. PNCB500KS)