DNA polymerase III synthesizes DNA using the RNA primer made by the DNA primase at the DNA replication fork of bacteria. DNA polymerase III alpha is the catalytic subunit of the polymerase. The DNA Polymerase Assay Kit is based on measurement of the DNA molecules synthesized by the DNA polymerase. The assay can be performed in 96-well plate or 384-well plate format for high throughput screening of DNA polymerase inhibitors.
Each kit includes the assay buffer and DNA template for 100 assays of DNA polymerase reactions in a 96-well plate format or 200 assays in a 384-well assay format. Enzyme is not included. The assay conditions are optimized for bacterial DNA polymerase III alpha subunit.
Each kit includes the assay buffer and DNA template for 100 assays of DNA polymerase reactions in a 96-well plate format or 200 assays in a 384-well assay format. E. coli DNA polymerase III alpha is included.
