Membrane Protein Extraction Tools

Purification of membrane proteins requires solubilization (extraction) of the proteins from the cell membranes.  The common problems are (1) the interested membrane protein is not efficiently extracted from the membranes or (2) the protein is extracted in an inactive form.  ProFoldin’s Membrane Protein Extraction Solutions provide optimal conditions for efficient extraction and stabilization of membrane proteins from the cell membranes by using a variety of non-denaturing detergent and synthetic lipid analogs including alkyl saccharides, acyl-N-methylglucamide, bile acid salt, alkylaminoxide, alkylpolyethylenes, zwitterionic detergents, and synthetic phosphocholine derivatives.  See the following detergent list in Membrane Protein Extraction Solutions.

The Membrane Protein Extraction Kit (MPE01-12S) includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.

The Membrane Protein Extraction Kit Plus (MPE01-12P) includes 5 ml of 12 solutions for screening and middle-scale preparative extraction of membrane proteins (up to 16 mg) from the cell membranes.

The Membrane Protein Extraction Solution (MPE01 to MPE12) provides 40 ml of a specific solution for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

                    MPE01  (Solution #1)     MPE02  (Solution #2)

                    MPE03  (Solution #3)     MPE04  (Solution #4)

                    MPE05  (Solution #5)     MPE06  (Solution #6)  

                    MPE07  (Solution #7)     MPE08  (Solution # 8)    

                    MPE09  (Solution #9)     MPE10  (Solution #10)  

                    MPE11  (Solution #11)   MPE12  (Solution #12)

 

      Detergent list in the Membrane Protein Extraction

      Solutions:

Alkyl saccharides                          MPE01, MPE02

Acyl-N-methylglucamide                MPE04

Bile acid salt                                 MPE06

Alkylaminoxide                             MPE07

Alkylpolyethylenes                        MPE03, MPE05, MPE11

Zwitterionic detergents                  MPE08, MPE09, MPE12

Phosphocholine derivative              MPE10

 

References:

  1. Bonander N et al, Production, purification and characterization of recombinant, full-length human claudin-1, PLoS One. 8(5):e64517 (2013).
  2. Welsh MJ et al, Transgenic pig models of cystic fibrosis, US patent 20140255975A1, Sep 11, 2014.
  3. Vega MC, Advanced Technologies for Protein Complex Production and Characterization. Springer Cham Heidelberg, New York. p148, (2016).