Purification of membrane proteins requires solubilization (extraction) of the proteins from the cell membranes.  The common problems are (1) the interested membrane protein is not efficiently extracted from the membranes or (2) the protein is extracted in an inactive form.  ProFoldin’s Membrane Protein Extraction Solutions provide optimal conditions for efficient extraction and stabilization of membrane proteins from the cell membranes by using a variety of non-denaturing detergent and synthetic lipid analogs including alkyl saccharides, acyl-N-methylglucamide, bile acid salt, alkylaminoxide, alkylpolyethylenes, zwitterionic detergents, and synthetic phosphocholine derivatives.  See the following detergent list in Membrane Protein Extraction Solutions.

The Membrane Protein Extraction Kit (MPE01-12S) includes 0.5 ml of 12 solutions for screening and micro-scale preparative extraction of membrane proteins (up to 1.5 mg) from the cell membranes.

The Membrane Protein Extraction Kit Plus (MPE01-12P) includes 5 ml of 12 solutions for screening and middle-scale preparative extraction of membrane proteins (up to 16 mg) from the cell membranes.

The Membrane Protein Extraction Solution (MPE01 to MPE12) provides 40 ml of a specific solution for large-scale preparative extraction of membrane proteins (up to 130 mg) from the cell membranes.

                    MPE01  (Solution #1)     MPE02  (Solution #2)

                    MPE03  (Solution #3)     MPE04  (Solution #4)

                    MPE05  (Solution #5)     MPE06  (Solution #6)  

                    MPE07  (Solution #7)     MPE08  (Solution # 8)    

                    MPE09  (Solution #9)     MPE10  (Solution #10)  

                    MPE11  (Solution #11)   MPE12  (Solution #12)

      Detergent list in the Membrane Protein Extraction

      Solutions:

Alkyl saccharides                         MPE01, MPE02

Acyl-N-methylglucamide                MPE04

Bile acid salt                               MPE06

Alkylaminoxide                             MPE07

Alkylpolyethylenes                        MPE03, MPE05, MPE11

Zwitterionic detergents                 MPE08, MPE09, MPE12

Phosphocholine derivative              MPE10