The MicroMolar Universal Kinase Assay Kit is based on measurement of ADP generated form the kinase reaction. It is used to measure the activities of purified kinases producing 0.1 mM – 10 mM (micromolar) ADP. The assay is fluorescence-based and can be carried out using regular black or white 96-well or 384-well plates or micro-cuvettes. It is important to avoid using unnecessarily high ATP concentrations in the kinase assays. There are major two reasons for this. One is to allow the assay to detect ATP-competitive inhibitors. The other is to avoid a high ADP background from ADP contamination in the ATP samples. It is recommended to use an ATP concentration in the range of 10 mM to 50 mM (micromolar).

MicroMolar Universal Kinase Assay Kit (enzyme not included) (Catalog Number: MUK100K) The kit contains the reaction buffer, ATP, MUK Reagent A, MUK Reagent B and fluorescencedye. The kit reagents are sufficient for 100 assays of a purified kinase using a standard black 384-well plate (Matrix 4318). 

Thymidylate Kinase Assay Kit Plus (enzyme included) (Catalog number: TMK100KE) The kit contains the reaction buffer, E. coli thymidylate Kinase, ATP, dTMP, MUK Reagent A, MUK reagent B and fluorescencedye. The kit reagents are sufficient for 100 assays of E. coli thymidylate kinase using a standard black 384-well plate (Matrix 4318). 

Human Thymidylate Kinase Assay Kit Plus (enzyme included) (Catalog Number: HTMK500KE) The kit contains the reaction buffer, human thymidylate kinase, ATP, dTMP, MUK Reagent A, MUK Reagent B and fluorescence dye.  The kit reagents are sufficient for 500 assays of human thymidylate kinase using a standard black 384-well plate (Matrix 4318).