UDP-N-acetylglucosamine pyrophosphorylase (GlmU) Assays
UDP-N-acetylglucosamine pyrophosphorylase (GlmU) is a bifunctional enzyme that catalyzes transfer of acetyl and uridyl groups onto glucosamine-1-P to generate UDP-GlcNAc, an essential precursor of peptidoglycans. UDP-GlcNAc is involved in the synthesis of the N-acetylglucosamine polysaccharide adhesin required for biofilm formation in bacteria.
The E. coli UDP-N-acetylglucosamine pyrophosphorylase (GlmU)Assay is based on measurement of the pyrophosphate generated from the GlmU reaction. The pyrophosphate is converted to phosphate by pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay can be used for screening inhibitors of E.coli GlmU in drug discovery research. It may also be used for characterization of E.coli GlmU.
E. coli GlmU Assay Kit Plus-100 (Cat # GLU100KE)
E. coli GlmU Assay Kit Plus-500 (Cat # GLU500KE)