Desalting columns

ProFoldin desalting columns are designed to remove small molecules like salts, enzyme substrates or ligands from a protein or DNA solution.  After desalting, the protein or DNA sample is in a low-salt buffer composed of 10 mM Tris-HCl, pH 7.5.  The columns can also be used for buffer exchange of protein or DNA samples to a desired buffer which is used to pre-equilibrate the column.  The principle of desalting is size-exclusion chromatography with a molecular cut-off of 5 kDa.  The residual salt concentration in the desalted solution is less than 2 % of the original salt concentration.  The protein recovery yield is 98 % or higher.

The Micro Desalting Spin Columns Set (Catalog number:  MDC050) contains 50 pre-packed spin-columns. Each column is to desalt 25 µl of sample. The volume of desalted sample is 25 to 30 µl.

The Preparative Desalting Column Set (Catalog number:  PDC020) contains 20 pre-packed preparative columns. Each column is to desalt 2.5 ml of sample. The volume of desalted sample is 3.5 ml.