The kit includes 300 µl of 10 x reaction buffer, 30 µl of 10 x DNA, 260 µl of SDS solution, 30 µl of 100 mM ATP, 26 µl of 100 x P. aeruginosa gyrase, 260 µl of proteinase K solutions, 10 ml of TDC matrix, 6 ml of rinse buffer, 500 µl of 10 x dye, a black receiver plate, a V-bottom reaction plate and a TDC filter plate.