The DNA Polymerase Assay Kit Plus is based on measurement of the DNA molecules synthesized by the DNA polymerase. The assay can be performed in 96-well plate or 384-well plate format for high throughput screening of DNA polymerase inhibitors. Each kit (Catalog number DPA100KE) includes the assay buffer, E. coli DNA polymerase III alpha subunit and DNA template for 100 assays of DNA polymerase reactions in a 96-well plate format or 200 assays in a 384-well assay format. The assay conditions are optimized for E. coli DNA polymerase III alpha subunit.