The human DNA polymerase beta assay is based on the principle that the repaired DNA forms more stable DNA duplex than the un-repaired one. The Assay Kit (catalog number DPB100K) is to measure the formation of the repaired DNA by its fluorescence signal in the presence of a fluorescence dye.  Each kit includes the assay buffer, DNA template and fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format.  The kit does not include human DNA polymerase beta that can be purchased from Trevigen (catalog number catalog number 4020-100-EB) or other commercial sources.