The E. coli DNA Primase Assay Kit is based on measurement of the RNA primers synthesized by the DNA primase in the presence of the DNA temperate and DNA helicase.   The assay can be performed in 96-well plate or 384-well plate format for high throughput screening of DNA primase inhibitors.  Each kit includes the assay buffer, DNA template and fluorescence dye for 100 assays of DNA primase reactions in a 96-well plate format or 200 assays in a 384-well assay format.  The assay conditions are optimized for E. coli DNA primase activity.