The Reagent Set is designed for drug discovery HTS uses.  Each reagent set includes the reaction buffer and fluorescence dye H19 for 1000 assays of DNA supercoiling reactions a 96-well plate assay format or 2000 assays in a 384-well assay format. Relaxed DNA is not included in the set.  Relaxed plasmid PBR322 from any commercial source can be used.