Beta Lactamase Assay

Resistance to β-lactam antibiotics such as penicillins, cephalosporins, cephamycins and carbapenems are due to hydrolysis of the four-atom ring known as β-lactam by β-lactamases (penicillinase) produced by Gram-negative bacteria. Inhibition of β-lactamases prevents bacterial degradation of beta-lactam antibiotics.  ProFoldin’s high throughput assay for β-lactamases is based on binding of the dye with the hydrolyzed β-lactam that generates fluorescence at 535 nm (excitation at 485 nm). The assay can be performed in a 384-well or 96-well plate format for tests of β-lactamase activities and throughput screening of inhibitors against β-lactamases.

The Beta Lactamase Assay Kit (Catalog No. LAC100K) includes 550 µl of 10 x Buffer and 110 µl of 50 x penicillin G and 260 µl of 10 x fluorescence dye. It is for 100 assays of β-lactamases in a 96-well plate or 384-well plate format. All assay reagents except the enzyme are included.

The E coli Beta Lactamase Assay Kit Plus (Catalog No. LAC100KE) includes 550 µl of 10 x Buffer and 110 µl of 50 x penicillin G, 260 µl of 10 x fluorescence dye and 55 µl of 100 x E. coliβ-lactamases. It is for 100 assays of β-lactamases in a 96-well plate or 384-well plate format.