Fluorescence-based topoisomerase assays in a 96-well or 384-well plate format are available for various topoisomerases from gram-negative and gram-positive bacteria.  These high throughput assays are suitable for screening topoisomerase inhibitors.

E. coli topoisomerase I assays

E. coli topoisomerase II (gyrase) assays

S. aureus topoisomerase II (gyrase) assays

E. coli topoisomerase IV (parC-parE complex) assays

S. aureus topoisomerase IV (parC-parE complex) assays

For other gram-negative and gram-positive bacterial topoisomerase assays, please contact ProFoldin.