The MicroMolar ADP Assay is for measurement of ADP concentrations in a sub-micromolar to low micromolar range. Other nucleoside diphosphates (UDP, GDP and CDP) showed very little background.  Nucleoside triphosphates (ATP, UTP, GTP and CTP) and monophosphates (AMP, UMP, GMP and CMP) are not detected. 

The ADP assay is based on fluorescence measurement with emission at 535 nm and excitation at 485 nm.  It is a high throughput assay using regular black 96-well or 384-well plates.  Micro-cuvettes may also be used for detection of the fluorescence signals. The assay can be used to measure contamination of ADP in ATP samples or monitor hydrolysis of ATP. It can also be used for measurement of kinase activities and other enzyme activities that generate ADP molecules.  The ADP assay is compatible with common reaction buffers with magnesium.  It is not compatible with samples containing high concentrations of DNA or RNA.

MicroMolar ADP assay kit - 100 assays (Catalog number: MAD100K): The kit contains 10 x buffer, 100 x MAD reagent 1, 100 x MAD reagent 2 and 10 x fluorescence dye.  The assay reagents are sufficient for measurement of 100 samples of ADP using a standard black 384-well plate. 

MicroMolar ADP assay kit - 500 assays (Catalog number: MAD500K): The kit contains 10 x buffer, 100 x MAD reagent 1, 100 x MAD reagent 2 and 10 x fluorescence dye.  The assay reagents are sufficient for measurement of 500 samples of ADP using a standard black 384-well plate.