Moloney murine leukemia virus (M-MLV) reverse transcriptase assay

The Moloney murine leukemia virus (M-MLV) causes lymphocytic leukemia in mice. M-MLV reverse transcriptase (M-MLV RT) synthesizes a complementary DNA strand from single-stranded RNA or DNA.  The M-MLV Reverse Transcriptase Assay is based on measurement of the DNA molecules synthesized by M-MLV RT.  The assay can be performed in a 384-well or 96-well plate format for tests of M-MLV reverse transcriptase activities and high throughput screening of inhibitors.

The M-MLV Reverse Transcriptase Assay Kit (Catalog number: MLV100K) includes 400 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100 assays of M-MLV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.   

The M-MLV Reverse Transcriptase Assay Plus(Catalog number: MLV100KE) includes all reagents in the M-MLV Reverse Transcriptase Assay Kit (Catalog number: MLV100K) plus the enzyme, 7 µl 500 x M-MLV RT.