HIV Reverse Transcriptase Assay

Infection of human immunodeficiency virus (HIV) causes acquired immunodeficiency syndrome (AIDS). The HIV reverse transcriptase (HIV RT) is an attractive drug target for HIV drug discovery. The HIV RT enzyme synthesizes a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The HIV Reverse Transcriptase Assay is based on measurement of the DNA molecules synthesized by HIV RT.  The assay can be performed in a 384-well or 96-well plate format for tests of HIV reverse transcriptase activities and high throughput screening of inhibitors.

The HIV Reverse Transcriptase Assay Kit (Catalog number: HIV100K) includes 400 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100 assays of HIV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.   

The HIV Reverse Transcriptase Assay Plus(Catalog number: HIV100KE) includes all reagents in the HIV Reverse Transcriptase Assay Kit (Catalog number: HIV100K) plus the enzyme, 7 µl 500 x HIV RT.