Avian myeloblastosis virus AMV reverse transcriptase assay

The avian myeloblastosis virus (AMV) is an alpha retrovirus responsible for acute myeloblastic leukemia (AML). AMV Reverse Transcriptase (AMV RT) catalyzes the synthesis of DNA using RNA or DNA template and dNTPs. The AMV Reverse Transcriptase Assay is based on measurement of the DNA molecules synthesized by AMV RT.  The assay can be performed in a 384-well or 96-well plate format for tests of AMV reverse transcriptase activities and high throughput screening of inhibitors.

The AMV Reverse Transcriptase Assay Kit (Catalog number: AMV100K) includes 400 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100 assays of AMV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.   

The AMV Reverse Transcriptase Assay Plus(Catalog number: AMV100KE) includes all reagents in the AMV Reverse Transcriptase Assay Kit (Catalog number: AMV100K) plus the enzyme, 7 µl 500 x AMV RT.